Biolegend, 408002, Purified anti-Allophycocyanin (APC) Antibody, 500μg

The APC003 antibody reacts with both allophycocyanin (APC) alone and APC conjugated with antibody or streptavidin, as well as APC tandem dyes such as APC/Cyanine5.5 and APC/Cyanine7. When APC003 antibody is used in a three-step staining procedure or separation of APC-antibody labelled cells, it neither quenches APC fluorescence nor reacts with tested mouse and human cells. The APC003 antibody can be used for separation of APC-antibody labeled cells or as a secondary step to amplify APC conjugated primary antibody signals (i.e. biotinylated APC003 as secondary step, followed by Streptavidin-APC).
500μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: allophycocyanin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Use at concentrations comparable to those of the primary antibody of interest. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Ma KY, et al. 2021. Nat Immunol. 22:1590. PubMed Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Janela B, et al. 2019. Immunity. 50:1069. PubMed Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed Matas-Rico E, et al. 2021. Cell Rep. 37:110013. PubMed Bender C, et al. 2020. Sci Adv. :6. PubMed
RRID: AB_345357 (BioLegend Cat. No. 408001) AB_345358 (BioLegend Cat. No. 408002)
Gene ID: NA
UniProt: View information about Allophycocyanin on UniProt.org
Clone: APC003
Regulatory Status: RUO
Isotype: Mouse IgG1, κ