Biolegend, 408101, Purified anti-phycoerythrin (PE) Antibody, 50μg

The PE001 antibody reacts with both phycoerythrin (PE) alone and PE conjugated with antibody or streptavidin, as well as PE tandem dyes such as PE/Cyanine5, PE/Cyanine5.5 and PE/Cyanine7. When PE001 antibody is used in a three-step staining procedure or separation of PE-antibody labeled cells, it does not quench PE fluorescence or react with tested mouse and human cells. The PE001 antibody can be used for separation of PE-antibody labeled cells or as a secondary step to amplify PE conjugated primary antibody signals (i.e. biotinylated PE001 as secondary step, followed by Streptavidin-PE).
50μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Phycoerythrin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Use at concentrations ≤ 0.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Taylor RT, et al. 2007. J. Immunol. 178:5659.
Product Citations: Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed Ma KY, et al. 2021. Nat Immunol. 22:1590. PubMed Dolton G, et al. 2022. Cell. 185:2936. PubMed Kwon M, et al. 2020. Clin Cancer Res. 1644:26. PubMed Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed Scur M, et al. 2022. Nat Commun. 13:7272. PubMed Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed Janela B, et al. 2019. Immunity. 50:1069. PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed Rius C, et al. 2018. J Immunol. 200:2263. PubMed Smith CC, et al. 2019. Cancer Immunol Res. 1.396527778. PubMed Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
RRID: AB_389236 (BioLegend Cat. No. 408101) AB_2168924 (BioLegend Cat. No. 408102)
Gene ID: NA
UniProt: View information about Phycoerythrin on UniProt.org
Clone: PE001
Regulatory Status: RUO
Other Names: PE
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.