Biolegend, 408104, Biotin anti-phycoerythrin (PE) Antibody, 100μg

The PE001 antibody reacts with both phycoerythrin (PE) alone and PE conjugated with antibody or streptavidin, as well as PE tandem dyes such as PE/Cyanine5, PE/Cyanine5.5 and PE/Cyanine7. When PE001 antibody is used in a three-step staining procedure or separation of PE-antibody labeled cells, it does not quench PE fluorescence or react with tested mouse and human cells. The PE001 antibody can be used for separation of PE-antibody labeled cells or as a secondary step to amplify PE conjugated primary antibody signals (i.e. biotinylated PE001 as secondary step, followed by Streptavidin-PE).
100μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Phycoerythrin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Use at concentrations ≤ 0.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Taylor RT, et al. 2007. J. Immunol. 178:5659.
Product Citations: Cacialli P, et al. 2022. EMBO J. 41:e108536. PubMed
RRID: AB_2237499 (BioLegend Cat. No. 408103) AB_2168923 (BioLegend Cat. No. 408104)
Gene ID: NA
UniProt: View information about Phycoerythrin on UniProt.org
Clone: PE001
Regulatory Status: RUO
Other Names: PE
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.