Biolegend, 408304, Biotin anti-FITC Antibody, 100μg

The FIT-22 antibody reacts with both fluorescein isothiocyanate (FITC) alone and FITC conjugated with antibody or streptavidin. When FIT-22 antibody is used in a three-step staining procedure or separation of FITC-antibody labeled cells, it does not quench FITC fluorescence or react with tested mouse and human cells. The FIT-22 antibody can be used for separation of FITC-antibody labeled cells or as a secondary step to amplify FITC conjugated primary antibody signals (i.e. biotinylated FIT-22 as secondary step, followed by Streptavidin-FITC).
100μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: FITC conjugated mouse IgG.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Bhadra R, et al. 2013. Infect Immun . 81:3414. PubMed Zhao L, et al. 2020. Pharm Biol. 1085:58. PubMed Hwang S, et al. 2016. J Exp Med. 213: 1799 - 1818. PubMed
RRID: AB_528899 (BioLegend Cat. No. 408304)
Gene ID: NA
UniProt: View information about FITC on UniProt.org
Clone: FIT-22
Regulatory Status: RUO
Other Names: Fluorescein isothiocyanate
Isotype: Mouse IgG1, λ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.