Biolegend, 408611, PerCP/Cyanine5.5 anti-mouse IgM, 25μg

This antibody was raised by immunization with IgM a mAb TC31. It specifically reacts with mouse IgM of Igh-C a and the related haplotypes (c, f, g, h, and j). It has been reported that beads coated with MA-69 antibody can stimulate B cell proliferation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, stimulation of B cell proliferation, and immunohistochemistry of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Sieckmann D, et al. 1991. Hybridoma 10:121. Stall AM. 1996. Weir's Handbook of Experimental Immunology pp. 27.
RRID: AB_2728435 (BioLegend Cat. No. 408611) AB_2728436 (BioLegend Cat. No. 408612)
Gene ID: 16019
UniProt: View information about IgMa on UniProt.org
Clone: MA-69
Regulatory Status: RUO
Other Names: Igh-6a
Isotype: Mouse (C57BL/6) IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.