Biolegend, 408903, Biotin anti-rat IgM Antibody, 100μg

The MRM-47 monoclonal antibody reacts with rat immunoglobulin M (IgM) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRM-47 antibody can be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.
100μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mixed rat Igs
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested IHC-F - Verified ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 2.5 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Li K, et al. 2022. Cancer Cell. 40:1374. PubMed Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed Muppidi J, et al. 2011. J Exp Med. 208:1941. PubMed Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
RRID: AB_10571208 (BioLegend Cat. No. 408903)
Gene ID: 299357
UniProt: View information about IgM on UniProt.org
Clone: MRM-47
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Mouse IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.