Biolegend, 409004, PE anti-Biotin Antibody, 100μg

Biotin (vitamin B7 or vitamin H) is essential for cell growth and the metabolization of fat and protein. It is also often used to tag a molecule of interest. The anti-biotin antibody can be used as a second-step reagent for detecting biotinylated targets.
100μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Biotinylated limulus hemocyanin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for relevant formats) include: immunoprecipitation and ELISA.
Application References(PubMed link indicates BioLegend citation): Uccellini MB, et al. 2008. J. Immunol. 181:5875. Green NM, et al. 2012. J Biol Chem. 287:39789. PubMed.
Product Citations: Hossain MA, et al. 2022. Cell Rep. 39:111021. PubMed Guo H, et al. 2022. Int J Oncol. 61: . PubMed Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed Tatovic D, et al. 2015. J Immunol. 195: 386 - 392. PubMed Howe EN, et al. 2020. Nat Commun. 2.553472222. PubMed Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed Chan W, et al. 2014. Leukemia. . PubMed Nakatsugawa M, et al. 2016. Sci Rep. 6:23821. PubMed Zou F, et al. 2019. Nat Commun. 10:4109. PubMed
RRID: AB_10645482 (BioLegend Cat. No. 409003) AB_10641847 (BioLegend Cat. No. 409004)
Ligand/Receptor: Avidin, Streptavidin
Gene ID: NA
UniProt: View information about Biotin on UniProt.org
Clone: 1D4-C5
Regulatory Status: RUO
Other Names: Vitamin B7, Vitamin H
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.