Biolegend, 410902, HRP Donkey anti-human IgG (minimal x-reactivity) Antibody, 500microl

This polyclonal donkey anti-human IgG antibody reacts with the heavy chains of human IgG and with the light (kappa and lambda) chains common to most human immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, bovine, horse, and rabbit immunoglobulins, but it may cross reaction with other subclasses of human immunoglobulins.
500microl
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Donkey
Formulation: Aqueous buffered solution containing a carrier protein and preservatives.
Preparation: The polyclonal antibody was purified from donkey antiserum by human immunoglobulin affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA. Do not use this product beyond the expiration date. For ELISA, the reagent should be titrated between 1:1,000 - 1:20,000 to determine optimal conditions. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase.
Application Notes: Cross-reactivity with the light chains of other human immunoglobulins, e.g. IgE and IgM.
Product Citations: Tsai DY, et al. 2023. Vaccines (Basel). 11:. PubMed Williams DM, et al. 2023. iScience. 26:107056. PubMed Sun X, et al. 2022. Appl Environ Microbiol. 88:e0230321. PubMed Biering SB, et al. 2022. Nat Commun. 13:7630. PubMed Escobar A, et al. 2022. Front Immunol. 12:766278. PubMed Bosshard N, et al. 2021. Rheumatol Ther. 8:1043. PubMed Gonzales SJ, et al. 2022. Front Immunol. 13:809264. PubMed
RRID: AB_2686937 (BioLegend Cat. No. 410902)
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3492
UniProt: View information about IgG on UniProt.org
Clone: Poly24109
Regulatory Status: RUO
Isotype: Donkey Polyclonal Ig