Biolegend, 420514, Cell-Vive™ CD RBC Lysis Buffer (10X), GMP, 200ml

Cell-Vive™ CD RBC Lysis Buffer 10X, GMP is a chemically-defined buffer designed to insure optimal lysis of human red blood cells (RBC) with minimal effects on leukocytes. This product is formulated without animal-derived components or preservatives, contains low levels of endotoxin (< 1EU/mL), and is tested negative for mycoplasma and microbial growths. Cell-Vive™ CD RBC lysis Buffer 10X, GMP is a 10X solution that should be diluted to 1X before use. This GMP product is for research and further ex vivo bioprocessing use only. BioLegend Cell-Vive™ GMP cell culture products are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include
200ml
Formulation: Chemically-Defined, no preservatives. Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X solution that should be diluted to 1X using water
Endotoxin Level: < 1 EU/mL
Preparation: Chemically-Defined, no preservatives. Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X solution that should be diluted to 1X using water. Warm the 1X solution to room temperature before use.
Storage & Handling: Store between 2°C-8°C
Recommended Usage: Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X solution that should be diluted to 1X using water. 2 ml of 1X Cell-Vive™ CD RBC Lysis Buffer, GMP is enough to lyse up to 100 μl of whole blood.
Application Notes: Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is recommended for the lysis of human red blood cells. This buffer has a chemically-defined formulation without animal-derived components, it contains low levels of endotoxin (< 1EU/mL), and it is tested negative for mycoplasma and microbial growths. Use Cell-Vive™ CD RBC Lysis Buffer (10X), GMP for highly sensitive assays. Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X formulation that contains no preservatives, handle under aseptic conditions as needed.
Additional Product Notes: Human Red Blood Cells lysis protocol Dilute the Cell-Vive™ CD RBC Lysis Buffer (10X), GMP to 1X working concentration with deionized water. Warm the 1X solution to room temperature before use. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 μl of human whole blood. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature for 10-15 minutes. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing the pellet. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell-Vive™ CD Cell Staining Buffer, GMP Cat. No. 420508), and wash 1X. Resuspend and proceed with further procedures.
Disclaimer: BioLegend Cell-Vive™ GMP Cell Culture products are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Gene ID: NA
Other Names: Red blood cell lysis buffer, lysis buffer, CD buffer, RBC Buffer