Biolegend, 421944, ATP Red, 1mg

ATP Red is a cell-permeable ATP sensor that localizes to mitochondria and produces fluorescence upon binding to ATP, rapidly responding to fluctuations in mitochondrial ATP levels. It provides a direct and simplified approach to measuring ATP levels in live cells by flow cytometry or live cell imaging. ATP Red allows for the discrimination of rare sub-populations while interrogating important cellular metabolic pathways, such as glycolysis and oxidative phosphorylation. This method is rapid – results can be obtained in less than an hour and yields an accurate measurement of cellular ATP production and its dependency on metabolic pathways of interest.
1mg
Verified Reactivity: Human, Mouse
Formulation: 1 vial of lyophilized ATP Red, 1 mg
Preparation: Bring vial to room temperature. To make a 10 mM stock solution, add 178 µL of DMSO to the vial of 1 mg ATP Red and mix thoroughly until completely dissolved. Note: Store unused ATP Red stock solution in a tightly sealed vial in the dark at room temperature. Stock solution is stable for at least 12 months.
Storage & Handling: 2 - 8°C
Application: FC - Quality tested Live cell imaging - Verified
Recommended Usage: Please refer to the detailed protocol in the application notes section.
Application Notes: Components: 1 vial of 1 mg lyophilized ATP Red, Molar Mass: 561.48 g/mol Storage: Upon receipt, store lyophilized probe at 2 - 8°C and protected from light. Upon reconstitution, store unused ATP Red stock solution in a tightly sealed vial in the dark at room temperature. Stock solution is stable for at least 12 months. Imaging Guidelines: Ex/Em = 590/620 nm Fluorescence microscope filter set: Cyanine3/TRITC Flow Guidelines: Analysis in PE or similar channel (585/42 or 575/25 or 570/40 filter set) Protocol: Grow cells in a tissue culture vessel with a coverslip bottom in desired media or prepare cell suspension containing 0.25 - 2.5 x106 cells in 100 - 500 µL of desired media or Cell Staining Buffer (Cat. No. 420201). Optional: Fix cells with Fixation Buffer (Cat. No. 420801), if desired. Add ATP Red at a final concentration of 5 - 10 µM along with desired antibodies. Incubate cells at room temperature or 37°C for 15 minutes (or up to 30 minutes for live cell imaging). Optional: Wash cells with PBS or equivalent buffer. If only staining with ATP Red, this washing step can be skipped. Proceed directly to analysis.
Distribution: Mitochondria
Biology Area: Adaptive Immunity, Cell Biology, Cell Proliferation and Viability, Immunology, Innate Immunity, Mitochondrial Function
Molecular Family: Mitochondrial Markers
Antigen References: Wang L, et al. 2016. Angew Chem Int Ed Engl. 55:1773-6.
Gene ID: NA
Regulatory Status: RUO