Biolegend, 422305, Human TruStain FcX™, GMP, 50tests

Human Fc receptors (FcRs) are expressed on a variety of cells, such as monocytes, granulocytes, B cells and dendritic cells. The cells with FcR expression sometimes give false positive or false negative results of immunofluorescent staining due to the FcRs-mediated Ig Fc binding. Human TruStain FcX™ is specially formulated for blocking the FcR-involved unwanted staining without interfering with antibody-mediated specific staining of human cells. Human TruStain FcX™ is compatible with flow cytometric staining with anti-human CD16 (clone 3G8), CD32 (clone FUN-2), and CD64 (clone 10.1) antibodies.
50tests
Formulation: Human IgG in PBS containing 0.09% sodium azide
Storage & Handling: Store between 2°C-8°C
Application: FC - Quality tested
Recommended Usage: It is recommended to add 5 µl of Human TruStain FcX™ per million cells in 100 µl staining volume, mix and incubate at room temperature for 5-10 minutes prior to staining with antibody of interest. It is not necessary to wash cells between these blocking and immunostaining steps.
Application Notes: This buffer contains specialized human IgG in PBS containing 0.09% sodium azide. It is not recommended to be used for staining human IgG. Handle as biohazard agent.
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Molecular Family: Fc Receptors
Gene ID: NA
Other Names: Fc Blocker, FcγR blocker, Fc inhibitor