Biolegend, 422401, RBC Lysis/Fixation Solution (10X), 50ml

BioLegend RBC Lysis/Fixation Solution has been designed and optimized for one-step lysing of red blood cells (RBCs) and fixation  of the remaining blood elements (such as leukocytes) following immunofluorescent staining with fluorochrome-conjugated antibodies. It can be used for either lyse/wash or lyse/no wash immunofluorescent staining procedures. This solution is supplied as a 10X concentrate. Dilute to 1X working solution with room temperature dI H 2 0 prior to use.
50ml
Formulation: This RBC Lysis/Fixation Solution is supplied as a 10X solution and should be diluted to 1X in room temperature deionized water.
Preparation: The pH of the 1X solution should fall within the range of pH 7.1 - 7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage & Handling: Store RBC lysis buffer between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Whole Blood Lyse/Wash Procedure: 1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution with 9 parts of room temperature dI H20. 2. Mix 100 µl of whole blood with fluorochrome-conjugated antibody. 3. Incubate at room temperature, in the dark, for 20 minutes. 4. Add 2.0 mL 1X RBC Lysis/Fixation solution, gently mixing each tube immediately. 5. Incubate at room temperature, in the dark, for 15 minutes. 6. Centrifuge at 350Xg for five minutes. Discard supernatant. 7. Add 2 mL BioLegend Cell Staining Buffer (Cat No 420201). 8. Centrifuge at 350Xg for five minutes. Discard supernatant. 9. Resuspend cell pellet in 0.3-0.5 mL Cell Staining Buffer. 10. Analyze cells immediately by flow cytometry or store at 4°C, in the dark, for no longer than 12 hours. Whole Blood Lyse/No Wash Procedure: 1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution with 9 parts room temperature dI H20. 2. Mix 50 µl of whole blood with fluorochrome-conjugated antibody. 3. Incubate at room temperature, in the dark, for 20 minutes. 4. Add 0.45 mL 1X RBC Lysis/Fixation solution, gently mixing each tube immediately. 5. Incubate at room temperature, in the dark, for 15 minutes. 6. Analyze samples by flow cytometry.
Application Notes: The 1X Lysis/Fixation solution can be used within 1 month from the date of preparation. It can be stored at room temperature or between 2°C and 8°C.
Application References(PubMed link indicates BioLegend citation): Horvath P, et al. 2012. J Infect Dis. PubMed
Product Citations: Kusner L, et al. 2014. PLoS One. 9:102231. PubMed Curry A, et al. 2017. PLoS One. 12(2):e0171586. PubMed Nerdal P, et al. 2016. J Immunol. 197: 3059 - 3068. PubMed Lee J, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.07.014. PubMed Horváth P, et al. 2012. J Infect Dis. 205:923. PubMed Foster G, et al. 2015. J Immunol. 195: 5380 - 5392. PubMed Camponova P, et al. 2017. Can J Physiol Pharmacol. 10.1139/cjpp-2016-0710. PubMed Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed Javed A, et al. 2015. PLoS One. 10: 0142086. PubMed
Gene ID: NA
Regulatory Status: RUO
Other Names: Red blood cell lysing solution