Biolegend, 422608, Human CD3/CD28/CD137 T Cell Activation Beads, VO22

The Human CD3/CD28/CD137 T Cell Activation Beads allow for convenient in vitro activation of human T cells without the need for antigen-presenting cells or precoating culture plates. Human T cells are stimulated with microbeads coated with monoclonal anti-CD3, anti-CD28 and anti-CD137 antibodies. The antibodies present on the bead surface act as an agonist and stimulate T cells. This mimics antigen presenting cells to induce the proliferation, especially for memory T cells which rely on CD137 signaling for cell maintenance and activation. Activation in vitro with this product results in robust T cell expansion, especially of the central memory (CCR7 + CD45RA - ) CD8 + T cell subset. Simply add the beads directly to isolated T cells at a 1:1 particle-to-cell ratio for robust T cell activation. The antibody-coated beads can be removed from the cells using a magnetic separator such as the MojoSort™ Magnet (Cat. Nos. 480019, 480020, and 480173). Some of the downstream applications include functional assays, gene expression analysis, and phenotypic characterization.
VO22
Verified Reactivity: Human
Formulation: Cocktail: N/A Particle: Phosphate buffer solution containing 0.1% HSA (azide-free). The particles are at a concentration of 4 x 107 particles/mL. The Human CD3/CD28/CD137 T Cell Activation Beads are tested for microbial/mycoplasma contamination.
Endotoxin Level: Less than 10 EU/mL as determined by the LAL test.
Preparation: The antibodies were purified by affinity chromatography and conjugated with magnetic microbeads under optimal conditions.
Storage & Handling: All components should be stored undiluted between 2°C and 8°C.
Application: Activ - Quality tested
Recommended Usage: Use Human CD3/CD28/CD137 T Cell Activation beads at a 1:1 particle-to-cell ratio.
Application Notes: This product is designed for the activation of isolated human T cells for research use only (RUO). The antibody-coated beads can be removed from the cells by the use of a handheld magnetic separator such as the MojoSort™ Magnet (Cat. Nos. 480019, 480020, and 480173) or other commercially available magnetic separators. Please contact BioLegend Technical Service for more product-specific information and guidance.
Gene ID: NA
Regulatory Status: RUO
Other Names: T Cell Stimulation Beads, Human T Cell Stimulation Beads, Human CD3 CD28 CD137 Beads, T cell expansion beads, Memory T cell Expansion Beads