Biolegend, 424101, DRAQ5™, 50microl

DRAQ5™ is a far-red emitting, anthraquinone compound that stains nuclei in live cells. It is permeant to live cells and thus can be used for cell cycle analysis and/or staining of nucleated cells. It is optimally excited at 568 nm, 633 nm, and 647nm and can be detected using 695LP, 715LP, and 780LP filters. DRAQ5™ can also be used in cell imaging and is a good replacement for DAPI, as it does not get excited by UV or Violet lasers and is sub-optimally excited by the 488 nm laser. DRAQ5™ can be combined with FITC, PE, and other UV or violet excitable dyes for multi-color analysis.
50microl
Preparation: DRAQ5™ is supplied at 50 µL per vial (5 mM).
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: DRAQ5™ should be stored between 2°C and 8°C upon receipt.
Application: FC, ICFC - Quality tested ICC, IHC-F, IHC-P - Verified
Recommended Usage: DRAQ5™ is a trademark of Biostatus Limited.
Application Notes: DRAQ5™ is a cell-permeant DNA binding anthraquinone dye that intercalates between A-T bases of dsDNA. Due to its cell permeability, this dye is useful for assessing DNA content and cell cycle but is not suitable to be used as a viability dye. In microscopy applications, DRAQ5™ is also useful as a nuclear counterstain for both live and fixed specimens. It is a far-red emitting dye with optimal excitation/ emission peaks of 633nm/ 695nm, respectively, (when intercalated into DNA) that can be detected in the Alexa Fluor® 647, APC and Alexa Fluor® 700 channels. For use in cell cycle analysis, the optimal concentration must be determined for each cell type. Also, due to the wide emission spectrum and the brightness of DRAQ5™ off the 633nm red laser and the dim emission intensity of the APC/Cyanine7 or APC/Fire™ 750 fluorophores, flow cytometric panels should be optimized to determine that including DRAQ5™ in a panel is compatible with the use of the APC/Cyanine7 or APC/Fire™ 750 channel as well. Protocol for DNA staining using DRAQ5™: 1. Perform surface staining following protocol of choice. 2. Wash cells twice with phosphate buffered saline (PBS). Sodium azide interferes with DRAQ5™ staining, thus it is recommended to stain in PBS (without calcium, magnesium, or sodium azide) or culture medium. 3. Dilute DRAQ5™ to required concentration. We recommend titrating the reagent to determine optimal concentration for cells of interest. Good results in flow cytometry and microscopy applications have been observed using a 1:200 to 1:1000 dilution. 4. Incubate at room temperature, preventing exposure to light, for 10-15 minutes. Subsequent washing can help make the cell cycle profile more distinct but is not absolutely necessary since DRAQ5™ is a fluorogenic reagent. 5. Analyze cells on a cytometer equipped with the 633 red laser. If performing cell cycle analysis, detection in the Alexa 700 channel with a 680LP or 715LP filter might help with resolution of the emission peaks.
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.
Product Citations: Monslow J, et al. 2020. Am J Pathol. 1118:190. PubMed Sándor N, et al. 2016. PLoS One. 11: 0163120. PubMed Wen J, et al. 2017. J Hematol Oncol. 10.1186/s13045-017-0489-9. PubMed Merlo LM, et al. 2020. Clin Pathol. 13:2632010X20951812. PubMed Webb A, et al. 2017. BMC Cancer . 10.1186/s12885-017-3418-y. PubMed Li Y, et al. 2020. Theranostics. 10:11376. PubMed
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Neuroscience
Antigen References: 1. Smith PJ, et al. 1999. J. Immunol. Methods. 229:131. 2. Smith PJ, et al. 2000. Cytometry. 40:280. 3. Yuan CM, et al. 2004. Cytometry B. Clin. Cytom. 58:47.
Gene ID: NA
Regulatory Status: RUO
Other Names: Live/Dead Staining, Dead cell exclusion dye, Cell viability dye