Biolegend, 424203, Flash Phalloidin™ Red 594, 300units

Molecular Mass 1535.79 g/mol.  Flash Phalloidin™ Red 594 excites maximally at 590 nm and emits maximally at 611 nm. Phalloidin is a bicyclic peptide that can be found naturally in the death cap mushroom.  This molecule is considered to bind so tightly to F-actin that when ingested by an organism, it will prevent the depolymerization of the actin polymeric filaments which leads to cellular toxicity.  In cell imaging, this is a very useful probe for imaging and stabilizing filamentous F-actin in fixed and permeabilized cells, providing structural and volumetric context to the cell.  Phallotoxins are conjugated to a wide array of fluorophores to enable their use in multicolor microscopy.
300units
Verified Reactivity: Human, Mouse, Rat, All Species
Preparation: Flash Phalloidin™ Red 594 is lyophilized. Reconstitute with 1.5mL of methanol to make a stock solution of 300 units.
Storage & Handling: Store Flash Phalloidin™ Red 594 at -20°C, protected from light.
Application: ICC - Quality tested IHC-F - Verified
Recommended Usage: Reconstitute the Flash Phalloidin™ Red 594 with 1.5mL of 100% methanol to make 300 units. Then prepare a working concentration by diluting 1:20 - 1:100 of stock in 1X PBS. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial. Fix cultured cells with 1% - 4% paraformaldehyde (PFA) for 10 minutes at room temperature. Wash the cells two times with 1X PBS. Permeabilize the cells with 0.5% Triton X-100 for 10 minutes at room temperature or at 4°C. Wash the cells two times with 1X PBS. Block cells with 5% fetal bovine serum for 30 minutes at room temperature. Prepare the working solution by diluting Flash Phalloidin™ Red 594 1:20 - 1:100 in 1X PBS. Stain the cells with diluted solution for 20 minutes at room temperature in the dark protected from light. Mount the slides with an antifade mounting media and image the slides.
Distribution: Cytoskeleton.
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience
Gene ID: NA
Regulatory Status: RUO
Other Names: Phalloidin