Biolegend, 425401, True-Phos™ Perm Buffer, 125ml

True-Phos™ Perm Buffer has been developed to permeabilize fixed peripheral blood mononuclear cells (PBMCs), lysed whole blood, cell lines, or splenocytes. Cells can be stimulated cytokines (e.g. IL-4, IL-6, etc.), antibodies (e.g. anti-CD3, clone HIT3a), or phorbol-12-myristate 13-acetate and ionomycin (Cell Activation Cocktail; Cat. No. 423301), and fixed with Fixation Buffer (Cat. No. 420801), or for whole blood, RBC Lysis/Fixation Solution (Cat. No. 422401). This buffer has been designed for use with antibodies for phospho-proteins (e.g. p-STAT6, p-ERK, etc.) and other intracellular targets (e.g. Cyclin B1, pan-STAT3, etc.). Not all cell surface markers are compatible with BioLegend’s True-Phos™ Perm Buffer.
125ml
Storage & Handling: Store between 2°C and 8°C, protected from prolonged exposure to light.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this product is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Use 1 mL of True-Phos™ Perm Buffer per 1 million cells, or 100 µL of whole blood.
Additional Product Notes: View more applications data using this product to stain Veri-Cells™ lyophilized control cells and to study phosphorylation kinetics in our Scientific Poster Library.
Product Citations: Ryan C, et al. 2016. Blood. 128(25):2899-2908. PubMed
Biology Area: Cell Biology, Signal Transduction, Transcription Factors
Molecular Family: Phospho-Proteins
Gene ID: NA
Regulatory Status: RUO
Other Names: Phospho-protein Fixation/Permeabilization Buffer Set