Biolegend, 426202, Lymphopure™, 500ml

Lymphopure™ is a ready-to-use filtered and endotoxin tested solution for the isolation of mononuclear cells (MNCs) from a cell suspension. The solution is formulated at a density that allows separation of MNCs from other cells in blood such as granulocytes and erythrocytes. Granulocytes and erythrocytes will sediment after centrifugation.
500ml
Formulation: Density gradient medium for the isolation of mononuclear cells formulated at a density of 1.077 ± 0.001 g/mL, and osmolality of 290 ± 15 mOsm.
Storage & Handling: Upon receipt, store at room temperature protected from light.
Application: Cell Separation - Quality tested
Recommended Usage: Watch a video protocol. Collect blood into a tube containing anti-coagulant (EDTA, heparin) Dilute the blood with an equal volume of phosphate-buffered saline or similar suitable medium. Mix Lymphopure™ by inverting the bottle several times. Aliquot half the amount of Lymphopure™ into a tube. For every ml of diluted blood, add 0.5 ml of Lymphopure™. Carefully layer the diluted blood on top of Lymphopure™. Avoid mixing blood with Lymphopure™. Centrifuge at 800 x g for 20 minutes at room temperature (15 - 25°C) without braking. If the blood has been stored for more than 2 hours, centrifuge for 30 minutes. After centrifugation, mononuclear cells (MNCs) form a defined cell layer at the plasma: Lymphopure™ interface. Collect the MNC layer at the interface without disturbing the other fractions. Wash MNCs with medium.
Application References(PubMed link indicates BioLegend citation): Böyum A. 1968. Scand. J. Clin. Lab. Invest. Suppl. 97:9-29. Böyum A. 1968. Scand. J. Clin. Lab. Invest. Suppl. 97:77-89. Böyum A. 1964. Nature. 204:793-4. Thorsby E, Bratile A. 1970. Histocompatibility Testing 1970,ed. P.I. Terasaki. 655-6. Ting A, Morris PJ. 1971. Vox. Sang. 20(6)561-3.
Gene ID: NA
Regulatory Status: RUO
Other Names: Density gradient medium