Biolegend, 430108, LEGEND MAX™ Human IFN-γ ELISA Kit, 5precoatedplates

Human IFN-γ (Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-γ, IFN-gamma, etc.) is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on its anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. Natural IFN-γ is a dimeric protein with subunits of 143 amino acids. IFN-γ is synthesized as a precursor protein of 166 amino acids including a secretory signal sequence of 23 amino acids. The protein is glycosylated. BioLegend’s LEGEND MAX™ Human IFN-γ ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IFN-γ specific monoclonal antibody is pre-coated on a 96-well strip-well plate. BioLegend’s LEGEND MAX™ Human IFN-γ ELISA Kit is specifically designed for the accurate quantitation of Human IFN-γ from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
5precoatedplates
Verified Reactivity: Human
Application: ELISA
Application References(PubMed link indicates BioLegend citation): Saito T, et al. 2014. Biochem Biophys Res Commun. 456:799. PubMed Johnston LR, et al. 2015. Atherosclerosis. 239:283. PubMed
Product Citations: Henault J, et al. 2015. Nat Immunol. 17: 196-203. PubMed Cavers A, et al. 2022. Ann Rheum Dis. 81:1603. PubMed Liu T, et al. 2021. Gut. 70:1965. PubMed Saito T, et al. 2014. Biochem Biophys Res Commun. 456:799. PubMed Zhou Z, et al. 2022. Front Pharmacol. 13:870178. PubMed Zeng W, et al. 2021. Exp Ther Med. 22:1186. PubMed Weist MR, et al. 2018. J Nucl Med. 59:1531. PubMed Park JJ, et al. 2021. Nat Commun. 12:1222. PubMed Johnston L, et al. 2015. Atherosclerosis. 239:283. PubMed Schiferle EB, et al. 2021. Sci Adv. 7: . PubMed Guo J, et al. 2019. Cancer Immunol Res. 1.349305556. PubMed Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed Zeng Y, et al. 2021. Aging (Albany NY). 13:. PubMed Do P, et al. 2020. ACS Comb Sci. 533:22. PubMed Damodharan SN, et al. 2020. Cytotherapy. 22:450. PubMed Brown CE, et al. 2018. Mol Ther. 26:31. PubMed
Sensitivity: 5.6 pg/mL
Standard Range: 15.6-1,000 pg/mL
Cell Sources: CD8+ and CD4+ T cells, NK cells
Biology Area: Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Gene ID: 3458
UniProt: View information about IFN-gamma on UniProt.org
Regulatory Status: RUO
Other Names: IFNG, IFG, IFI, interferon gamma, Immune interferon
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.