Biolegend, 430217, LEGEND MAX™ High Sensitivity Human TNF-α ELISA Kit, 1precoatedplate

Human Tumor Necrosis Factor α (TNF-α), also termed Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF), is a potent multifunctional cytokine that can exert regulatory, inflammatory, and cytotoxic effects on a wide range of normal lymphoid, non-lymphoid cells, and tumor cells. TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, NK cells, and many transformed cell lines. It exists as a soluble 17.5 kD protein and a 26 kD membrane-associated protein. The soluble protein form derives from the membrane form by way of proteolytic processing. The membrane form protein, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane occurs by binding to soluble TNFRSF1A/ TNFR1. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro , hemorrhagic necrosis of tumors in vivo , increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. It has been shown that TNF-α plays a critical role in many physiological and pathological conditions, including neurodegenerative diseases and cancer. The BioLegend LEGEND MAX™ High Sensitivity Human TNF-α ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a monoclonal mouse anti-human TNF-α capture antibody. The detection antibody is a biotinylated monoclonal mouse anti-human TNF-α antibody. This kit is specifically designed for the accurate quantitation of human TNF-α in serum, plasma, cell culture supernatant, and urine. This kit is analytically validated with ready-to-use reagents.
1precoatedplate
Verified Reactivity: Human
Application: ELISA
Sensitivity: 0.190 ± 0.092 pg/mL
Standard Range: 0.625 – 40 pg/mL
Molecular Family: Cytokines/Chemokines
Gene ID: 7124
UniProt: View information about TNF-alpha on UniProt.org
Regulatory Status: RUO
Other Names: Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF)
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.