Biolegend, 430926, FluoroSpot MAX™ Deluxe Set Mouse TNF-α, 5plates

TNF-α (Tumor Necrosis Factor α), also known as Cachectin, is a cytokine belonging to the Tumor Necrosis Factor superfamily. It is produced by various cell types, including macrophages, monocytes, neutrophils, T cells, NK cells, and epithelial cells. TNF-α can bind to two receptors: TNFR1 and TNFR2. TNF-α signaling plays a central role in the inflammatory response, lymphoid cell development, and lipid metabolism. Additionally, TNF-α is a pathological component in numerous autoimmune diseases, including but not limited to Crohn’s disease, asthma, and rheumatoid arthritis. It was also reported to be involved in some cancers and obesity. BioLegend’s FluoroSpot MAX™ Deluxe Set contains pre-optimized essential reagents, buffers, and solutions for the detection of Mouse TNF- α secreting cells. In this assay, anti-mouse TNF- α capture antibodies are coated on a clear-bottom microplate. Cells (stimulated or unstimulated) are added to the wells and incubated, allowing secreted TNF- α to bind to nearby capture antibodies. After incubation, cells are removed, and biotinylated detection antibodies are added followed by Streptavidin-PE. The resulting red spots represent the “footprint” of an individual TNF- α-secreting cell, allowing for precise quantification of cytokine-producing cells in the sample.
5plates
Verified Reactivity: Mouse
Application: FluoroSpot
Cell Type: Antigen-presenting cells, B cells, Granulocytes, Langerhans cells, Leukocytes, Lymphocytes, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells, NKT cells, Plasma cells, T cells, Tfh, Th1, Th17, Th2, Thymocytes, Tregs
Biology Area: Adaptive Immunity, Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Death, Immuno-Oncology, Immunology, Inhibitory Molecules, Innate Immunity, Mitochondrial Function, Signal Transduction, Ubiquitin/Protein Degradation
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, Cytokines/Chemokines, GTPases, Phospho-Proteins, Proteases, Protein Kinases/Phosphatase, Soluble Receptors, Toll Like Receptors, Tumor Suppressors
Gene ID: 21926
UniProt: View information about TNF-alpha on UniProt.org
Regulatory Status: RUO
Other Names: Tumor Necrosis Factor alpha, Cachectin, Tumor necrosis factor ligand superfamily member 2