Biolegend, 431317, LEGEND MAX™ High Sensitivity Mouse IL-6 ELISA Kit, 1precoatedplate

Mouse IL-6 (interleukin 6, also known as B-cell differentiation factor, B-cell stimulatory factor 2, interferon β-2, hybridoma growth factor, and cytotoxic T-lymphocyte differentiation factor) plays an essential role in the final differentiation of B-cells into immunoglobin-secreting cells. Also, it induces myeloma and plasmacytoma growth, promotes nerve cell differentiation, and (in hepatocytes) induces acute phase reactant proteins. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes. Current research suggests that IL-6 plays a critical role in many physiological and pathological conditions, including autoimmune diseases and rheumatoid arthritis. The BioLegend LEGEND MAX™ High Sensitivity Mouse IL-6 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a monoclonal anti-mouse IL-6 capture antibody. The detection antibody is a biotinylated monoclonal anti-mouse IL-6 antibody. After washing away any unbound biotinylated detection antibody, a streptavidin-polymer HRP is used for detection. Streptavidin-Polymer HRP enhances the sensitivity of the kit, allowing for detection of low concentrations of IL-6. This kit is specifically designed for the accurate quantitation of mouse IL-6 in serum, heparin plasma, and cell culture supernatant. This kit is analytically validated with ready-to-use reagents.
1precoatedplate
Verified Reactivity: Mouse
Application: ELISA
Sensitivity: 0.413 ± 0.148 pg/mL
Standard Range: 1.56 – 100 pg/mL
Molecular Family: Cytokines/Chemokines
Gene ID: 16193
UniProt: View information about IL-6 on UniProt.org
Regulatory Status: RUO
Other Names: Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor(HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.