Biolegend, 432008, LEGEND MAX™ Human GM-CSF ELISA Kit, 5precoatedplates

Granulocyte/macrophage - colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte and eosinophil progenitors. BioLegend’s LEGEND MAX™ Human GM-CSF ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human GM-CSF specific monoclonal antibody is pre-coated on a 96-well strip-well plate. BioLegend’s LEGEND MAX™ Human GM-CSF ELISA Kit is specifically designed for the accurate quantitation of Human GM-CSF from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
5precoatedplates
Verified Reactivity: Human
Application: ELISA
Application References(PubMed link indicates BioLegend citation): Samavedam UK, et al. 2014. J. Immunol. 192:559. PubMed
Product Citations: Samavedam U, et al. 2014. J Immunol. 192:559. PubMed
Sensitivity: 3.5 pg/mL
Standard Range: 15.6-1,000 pg/mL
Cell Sources: T cells, macrophages, fibroblasts, endothelial cells, mast cells
Biology Area: Cell Biology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Gene ID: 1437
UniProt: View information about GM-CSF on UniProt.org
Regulatory Status: RUO
Other Names: GM-CSF Pre-coated ELISA Kit
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.