Biolegend, 432307, LEGEND MAX™ Human Total IgG ELISA Kit, 1precoatedplate

IgG is a monomeric immunoglobulin composed of two identical heavy chains and two identical light chains linked together by inter-chain disulfide bonds. There are four IgG subclasses (IgG1, IgG2, IgG3, and IgG4) in humans named in order of decreasing abundance. They differ in the size of the hinge region and the number and arrangements of the interchain disulfide bonds linking their heavy chains. Of the 5 immunoglobulin isotypes, IgG is most abundant in human serum. IgG is synthesized and secreted by plasma B cells and is involved in the secondary immune response. The measurement of IgG can be a diagnostic tool for certain conditions such as autoimmune diseases, gastrointestinal conditions or immunoglobulin-deficiency disorders. The BioLegend LEGEND MAX™ Human Total IgG ELISA Kit is a Sandwich Enzyme- Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a human monoclonal anti-human IgG antibody. The detection antibody is a biotinylated anti-human IgG antibody. This kit is specifically designed for the accurate quantitation of human IgG in serum, plasma, breast milk, saliva and urine. This kit is analytically validated with ready-to-use reagents.
1precoatedplate
Verified Reactivity: Human
Application: ELISA
Sensitivity: 0.12 ± 0.03 ng/mL
Standard Range: 0.625 – 40 ng/mL
Cell Type: B cells
Biology Area: Adaptive Immunity, Immunology
Gene ID: NA
UniProt: View information about IgG on UniProt.org
Regulatory Status: RUO
Other Names: IgG, Immunoglobulin G, IgG1, IgG2, IgG3, IgG4
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.