Biolegend, 432908, LEGEND MAX™ Human Latent TGF-β ELISA Kit, 5precoatedplates

Latent TGF-ß is secreted as a complex with LAP and TGF-ß. LAP and TGF-ß1 are synthesized as single propeptide precursors of 390 amino acids with an N-terminal signal peptide of 29 amino acids, a 249 amino acids pro-region (LAP), and a 112 amino acids C-terminal region (TGF-ß1). Both LAP and TGF-ß1 exist as homodimers in circulation, but the disulfide linked homodimers of LAP and TGF-ß1 remain non-covalently associated, forming the small latent TGF-ß1 complex (SLC, 100 kD). The large latent TGF- ß1 complex (LLC, 235 – 260 kD) contains a third component, the latent TGF-ß binding protein (LTBP), which is linked to LAP by a single disulfide bond. LTBP does not confer latency but promotes efficient secretion of the complex to extracellular sites. TGF-ß and LAP can be released (activated) by many factors, including enzymes and low or high pH. TGF-ß and LAP have multiple functions, such as regulating proliferation and differentiation of various cell types. Increased production and activation of latent TGF-ß have been linked to immune defects associated with malignancy and autoimmune disorders, to susceptibility to opportunistic infection, and to the fibrotic complications associated with chronic inflammatory conditions. Latent TGF-ß has been found to protect against glomerulonephritis. It is a surface marker of activated regulatory T cells. LEGEND MAX™ Human Latent TGF-ß ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a capture antibody. This kit is specifically designed for the accurate quantitation of both small and large latent TGF-ß from cell culture supernatant, serum, plasma and other biological fluids. This kit is analytically validated with ready-to-use reagents.
5precoatedplates
Verified Reactivity: Human
Application: ELISA
Application Notes: Human Latent TGF-ß ELISA kit does not cross-react with bovine latent TGF-ß, and thus the measurement is not affected by FBS. This kit is suitable for FBS-containing samples such as culture supernatants.
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.
Product Citations: Werchau N, et al. 2022. Oncoimmunology. 11:2140534. PubMed Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed
Sensitivity: 0.23 ng/mL
Standard Range: 1.56-100 ng/mL
Cell Sources: Ubiquitously expressed by many kinds of cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family: Cytokines/Chemokines, Growth Factors
Gene ID: 7040
UniProt: View information about TGF-beta on UniProt.org
Regulatory Status: RUO
Other Names: Human Latent TGF-β Pre-coated ELISA Kit
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.