Biolegend, 433917, LEGEND MAX™ Human IL-17A ELISA Kit, 1precoatedplate

IL-17 or IL-17A originally identified as cytotoxic T lymphocyte-associated antigen-8 (CTLA-8) in mouse, is a founding member of the IL-17 family of cytokines. IL-17A is mainly produced by a small subset of T helper cells called Th17 cells. IL-17A is a 155 amino acid protein that is a disulfide linked, homodimeric, secreted glycoprotein with a molecular mass of 35 kD. The amino acid sequence of human IL-17A is 63% and 58% identical to mouse and rat IL-17A, respectively, and 72% identical to the ORF13 of Herpesvirus saimiri. IL-17A exhibits multiple biological activities including the induction of various proinflammatory cytokines and chemokines such as IL-6, IL-8, G-CSF, GM-CSF, GRO-a, and MCP-1 on a variety of cell types. IL-17A is also involved in the activation of NF-kB and costimulation of T cell proliferation. As a result of these roles, IL-17A has been linked to many immune/autoimmune related diseases including rheumatoid arthritis, asthma, lupus, and multiple sclerosis. BioLegend’s LEGEND MAX™ Human IL-17A ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is precoated with a capture antibody. This kit is specifically designed for the accurate protein quantitation of human IL-17A from cell culture supernatant, serum, plasma and other biological fluids. It is analytically validated with ready-to-use reagents.
1precoatedplate
Verified Reactivity: Human
Application: ELISA
Application References(PubMed link indicates BioLegend citation): Verma NK, et al. 2012. J. Biol Chem. 287:27204. PubMed.
Product Citations: Izati AF, et al. 2021. Front Immunol. 12:690908. PubMed Verma N, et al. 2012. J Biol Chem. 287: 27204. PubMed Moafi M, et al. 2017. Scand J Immunol. 85:291. PubMed Sun J, et al. 2019. Exp Ther Med. 1.752083333. PubMed Schwarzkopf K, et al. 2019. Hepatol Commun. 3:392. PubMed
Sensitivity: 0.8 pg/mL
Standard Range: 3.9-250 pg/mL
Cell Sources: Activated T cell subpopulation
Biology Area: Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Gene ID: 3605
UniProt: View information about IL-17A on UniProt.org
Regulatory Status: RUO
Other Names: IL-17A ELISA Kit, IL-17 ELISA Kit
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.