Biolegend, 436017, LEGEND MAX™ Human Tau (x-421) ELISA Kit, 1precoatedplate

Tau is a 441-amino acid protein that binds along microtubules in order to stabilize them. Phosphorylation of tau reduces its affinity for microtubules, leading to microtubule destabilization. Hyperphosphorylated tau can assemble into higher order structures, including oligomers and neurofibrillary tangles (NFT). NFTs are a hallmark of several neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), and frontotemporal dementia. In addition to phosphorylation, proteolytic cleavage of tau is an important post-translational modification. For example, caspase-3-mediated cleavage of tau at Asp421 is an early step in NFT formation and has been linked to an increase in neurotoxicity in AD patients. Furthermore, the number of NFTs composed of tau (x-421) is positively correlated with AD progression. Tau cleavage products have also been found in PD patients, thus demonstrating that truncated forms of tau could be a biomarker for multiple neurodegenerative diseases. The BioLegend LEGEND MAX™ Human Tau (x-421) ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a monoclonal mouse anti-human tau (x-421) capture antibody. The detection antibody is a biotinylated monoclonal mouse anti-human tau antibody. This kit is specifically designed for the accurate quantitation of human tau (x-421) in human cell/tissue lysates. This kit is analytically validated with ready-to-use reagents.
1precoatedplate
Verified Reactivity: Human
Application: ELISA
Sensitivity: 46.6 ± 11.2 pg/mL
Standard Range: 62.5 – 4000 pg/mL
Molecular Family: Tau
Gene ID: 4137
UniProt: View information about Tau on UniProt.org
Regulatory Status: RUO
Other Names: Tau-C, C-MAPT, Tau (1-421)
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.