Biolegend, 436708, LEGEND MAX™ Total TGF-β1 ELISA Kit, 5precoatedplates

Transforming growth factor beta 1 (TGF-β1) is a member of the transforming growth factor beta superfamily of cytokines. TGF- β1 precursor contains 390 amino acids with an N-terminal signal peptide of 29 amino acids required for secretion from a cell, a 249 amino acids pro-region ( latency associated peptide or LAP), and a 112 amino acids C-terminal region that becomes the active TGF- β1 upon activation. Both LAP and TGF- β1 exist as homodimers in circulation, but the disulfide linked homodimers of LAP and TGF- β1 remain non-covalently associated, forming the small latent TGFβ1 complex (SLC, 100 kD).  The large latent TGF- β1 Complex (LLC, 235 – 260 kD) contains a third component, the latent TGF- ß binding protein (LTBP), which is linked to LAP by a single disulfide bond.  The LTBP does not confer latency, but for efficient secretion of the complex to extracellular sites.  Free active TGF- β1 can be released (activated) by many factors including enzymes and low or high pH. TGF- β1 is nearly 100% conserved across mammalian species. It has diverse biological functions in multiple cellular processes such as regulating proliferation and differentiation of various cell types. TGF- β1 is also an important immunoregulatory cytokine, which is involved in the maintenance of self-tolerance, Th17 differentiation, and T cell homeostasis etc.
5precoatedplates
Verified Reactivity: Human, Mouse, Rat, Non-Human Primate, Pig, Cow, Rabbit
Application: ELISA
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Park GB, et al. 2014. Invest Opthalmol Vis Sci. 55:1770. PubMed Johannesson B, et al. 2014. Dis Model Mech. 7:977. PubMed
Product Citations: Ahangar P, et al. 2021. Wound Repair Regen. 29:380. PubMed Spinelli FM, et al. 2022. IUBMB Life. 74:927. PubMed Flaskamp L, et al. 2022. Cells. 11: . PubMed McNamara NB, et al. 2023. Nature. 613:120. PubMed Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed Sheng YR, et al. 2022. Cell Mol Life Sci. 79:173. PubMed Chen C, et al. 2016. J Lipid Res. 57: 1435 - 1446. PubMed Derakhshandeh R, et al. 2018. Oncotarget. 9:11126. PubMed Park G, et al. 2014. Invest Ophthalmol Vis Sci . 55:1770. PubMed Orgaz JL, et al. 2020. Cancer Cell. 37:85. PubMed Johannesson B, et al. 2014. Dis Model Mech. 7:977. PubMed Hancock MH, et al. 2020. Cell Host Microbe. 27:104. PubMed O’Conor C, et al. 2016. Sci Rep. 6:29053. PubMed Choi J, et al. 2017. Sci Rep. 7:43349. PubMed Qi S, et al. 2022. Transl Stroke Res. :. PubMed Tang R, et al. 2020. Exp Ther Med. 3.388194444. PubMed Strudwick XL, et al. 2020. Adv Wound Care (New Rochelle). 9:637. PubMed
Sensitivity: 3.5 pg/mL
Standard Range: 7.8-500 pg/mL
Cell Sources: Many cell types, highly expressed on activated Tregs and platelets
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family: Cytokines/Chemokines, Growth Factors
Gene ID: 21803
UniProt: View information about TGF-beta1 on UniProt.org
Regulatory Status: RUO
Other Names: Total TGF-β1 Pre-coated ELISA Kit
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.