Biolegend, 438205, ELISA MAX™ Deluxe Set Rat TNF-a, 10plates

Please contact our sales representative for bulk pricing quotations. TNF-α (Tumor Necrosis Factor α), also termed Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF), is a potent multifunctional cytokine which can exert regulatory, inflammatory and cytotoxic effects on a wide range of normal lymphoid, non-lymphoid cells, and tumor cells. TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells, and many transformed cell lines. It exists as a soluble 17.5 kD protein and a 26 kD membrane-associated protein. The soluble form protein derives from the membrane form by proteolytic processing.  Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro , hemorrhagic necrosis of tumors in vivo , increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.  It has been shown that TNF-α plays a critical role in many physiological and pathological conditions including neurodegenerative diseases and cancer. This kit is specifically designed for the accurate quantitation of Rat TNF-α from cell culture supernatant, serum, plasma, or other biological fluids.  It is designed to meet the increasing use of rat models in biomedical studies.
10plates
Verified Reactivity: Rat
Application: ELISA
Product Citations: Hajighorbani M, et al. 2017. Inflammopharmacology. 10.1007/s10787-017-0335-2. PubMed Wahba NS, et al. 2022. Fundam Clin Pharmacol. 36:306. PubMed da Silva TP, et al. 2022. Cell Biochem Funct. :. PubMed Asaad GF, et al. 2022. Iran J Basic Med Sci. 25:1522. PubMed Garcia-Cortadella R, et al. 2021. Nat Commun. 0.646527778. PubMed Schmidt K, et al. 2017. Microvasc Res. 10.1016/j.mvr.2017.08.002. PubMed Sanchez TW, et al. 2020. Am J Reprod Immunol. 83:e13205. PubMed van der Merwe Y, et al. 2019. Sci Rep. 9:3482. PubMed Lai PH, et al. 2021. J Neuroinflammation. 18:35. PubMed Murugesan P, et al. 2016. J Infect Dis. 213: 532 - 540. PubMed Niu Q, et al. 2022. Life (Basel). 12:. PubMed Lotter J, et al. 2020. Front Psychiatry. 11:121. PubMed
Sensitivity: 2 pg/ml
Standard Range: 15.6-1,000 pg/mL
Cell Sources: Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Gene ID: 24835
UniProt: View information about TNF-alpha on UniProt.org
Regulatory Status: RUO
Other Names: Tumor Necrosis Factor α (TNF-α) ELISA Kit, Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF)
Q: Do the ELISA MAX™ Deluxe Sets come with plates?
A: No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.