Biolegend, 438308, LEGEND MAX™ Human anti-mouse Ig (HAMA) ELISA Kit, 5precoatedplates

Human anti-mouse antibodies, also known as HAMA, are antibodies found in human serum which have the ability to bind to mouse immunoglobulin. Pre-existing HAMA activity has been detected in approximately 5-10% of normal populations, which is believed to be caused by exposure to mice or mouse agents. Therapeutic use of mouse monoclonal antibodies or products derived from mouse monoclonal antibodies are becoming the most common cause of HAMA activities in patient serum. There is an increasing need to determine the levels of HAMA in human serum samples in the therapeutic, assay development, and diagnostic areas. For example, in the therapeutic area, HAMA generated in response to injected monoclonal antibodies not only can bind and reduce the efficacy of the therapeutics, but also can cause anaphylactic complications to subsequent administration of mouse monoclonal antibodies. Therefore, it is useful for monitoring the prognosis of patients that received a mouse monoclonal antibody treatment. In addition, HAMA due to its nature to bind mouse IgG, tend to cause false positive results in Sandwich ELISA or false negative results in competitive immunoassays utilizing mouse monoclonal antibodies. It is important to measure serum samples for HAMA in case of unexpected immunoassay results. BioLegend’s LEGEND MAX™ Human Anti-Mouse Ig (HAMA) ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, mouse Ig is pre-coated on a 96-well strip-well plate. BioLegend’s LEGEND MAX™ Human Anti-Mouse Ig (HAMA) ELISA Kit is specifically designed for the accurate quantitation of Human anti-mouse Ig from serum and plasma. It is ready-to-use, accurate, and sensitive.
5precoatedplates
Verified Reactivity: Human
Application: ELISA
Product Citations: P, et al. 2016. Mol Ther. 24: 1602-1614. PubMed Gargett T, et al. 2016. Mol Ther. 10.1038/mt.2016.63. PubMed
Sensitivity: 1 ng/mL
Standard Range: 3.9-250 ng/mL
Biology Area: Immunology
Gene ID: NA
UniProt: View information about HAMA on UniProt.org
Regulatory Status: RUO
Other Names: Human anti-mouse antibodies (HAMA) Pre-coated ELISA Kit
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.