Biolegend, 439217, LEGEND MAX™ High Sensitivity Human Granzyme B ELISA Kit, 1precoatedplate

Granzymes are serine proteases that are released by cytoplasmic granules within cytotoxic T cells and natural killer (NK) cells. Human Granzyme B is one of the five members of the human granzyme family which includes Granzymes A, B, G, H, and K. Granzyme B is expressed as a 32 kD protein encoded by the GZMB gene. While Granzyme B is mostly expressed by CTL (Cytotoxic T Lymphocyte) and NK cells, it is also expressed in resting and activated plasmacytoid dendritic cells. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. Granzyme B and perforin have been shown to induce CTL-mediated target cell DNA fragmentation and apoptosis. Once released from the CTL, Granzyme B binds its receptor, the mannose-6-phosphate/insulin-like growth factor II receptor, and is endocytosed but remains arrested in endocytic vesicles until released by perforin. Once in the cytosol, Granzyme B targets caspase-3 and other caspases directly or indirectly through the mitochondria, initiating the caspase cascade which leads to DNA fragmentation and apoptosis. Elevated Granzyme B levels have been linked with various disease processes. The LEGEND MAX™ High Sensitivity Human Granzyme B ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with anti-human Granzyme B monoclonal antibody. The detection antibody is a biotinylated anti-human Granzyme B monoclonol antibody. This kit is specifically designed sensitively for the accurate quantitation of human Granzyme B in human serum, plasma, and cell culture supernatant. It is analytically validated with ready-to-use reagents.
1precoatedplate
Verified Reactivity: Human
Application: ELISA
Sensitivity: 0.63 ± 0.20 pg/mL
Standard Range: 1.88 - 120 pg/mL
Cell Type: Dendritic cells, Granulocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Enzymes and Regulators, Proteases
Gene ID: 3002
UniProt: View information about Granzyme B on UniProt.org
Regulatory Status: RUO
Other Names: Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, CTLA1, Serine Protease B, CSP-B, CSPB, Cytotoxic serine protease B, CCP1, Cytotoxic cell protease 1
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.