Biolegend, 439417, LEGEND MAX™ High Sensitivity Mouse IFN-β ELISA Kit, 1precoatedplate

Interferon-β (IFN-β) is a member of the type I interferon class. Mouse IFN-β is a ~20 kD monomeric glycoprotein that has 50% amino acid sequence homology with mouse IFN-α and 62% homology with human IFN-β. Type I IFNs are involved in the innate immune system, being released non-specifically during the early stage of infection in response to stimuli such as viruses, dsRNA, LPS, and bacterial components. Once secreted by virus-infected or activated cells, they bind to the IFNα/β receptor (IFNAR), which is found on almost all cell types. Then, they induce the expression of hundreds of IFN-stimulated genes (ISGs) via the JAK-STAT pathway. While the functions of many of these proteins remain to be elucidated, a hallmark of type I IFNs is their anti-viral activity. Type I IFNs also regulate cell growth and apoptosis, and they exert various immunomodulatory and inflammatory effects. As such, they are clinically relevant as therapeutics and as targets of inhibition for the treatment of various autoimmune diseases and cancer. IFN-β is currently the therapeutic of choice for treating multiple sclerosis. The BioLegend LEGEND MAX™ High Sensitivity Mouse IFN-β ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a polyclonal anti-mouse IFN-β capture antibody. The detection antibody is a biotinylated monoclonal anti-mouse IFN-β antibody. After washing away any unbound biotinylated detection antibody, a streptavidin-polymer HRP is used for detection. Streptavidin-Polymer HRP enhances the sensitivity of the kit, allowing for detection of low concentrations of IFN-β. This kit is specifically designed for the accurate quantitation of mouse IFN-β in serum, citrate plasma, heparin plasma, and cell culture supernatant. This kit is analytically validated with ready-to-use reagents.
1precoatedplate
Verified Reactivity: Mouse
Application: ELISA
Sensitivity: 0.247 ± 0.083 pg/mL
Standard Range: 0.94 – 60 pg/mL
Molecular Family: Cytokines/Chemokines
Gene ID: 15977
UniProt: View information about IFN-beta on UniProt.org
Regulatory Status: RUO
Other Names: Fibroblast interferon (Fi-IFN, F-IFN), Type-1 interferon, acid-stable interferon, R1-GI factor
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.