Biolegend, 439808, LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit, 5precoatedplates

Immunoglobulin E has long been regarded as a major molecular component of atopic diseases, including allergy and asthma. Asthma is a chronic respiratory disease characterized by bronchial inflammation and airway hyperresponsiveness. Clinical studies have found a close association between asthma and serum IgE levels. Allergen-specific IgE has been detected in nasal mucosa from allergic patient. Examination of murine models of human allergic asthma has provided novel insight into the pathobiologic events linking airway inflammation with airway dysfunction. Ovalbumin (OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin is an important protein in several different areas of research and commonly used to stimulate an allergic reaction in mice (e.g. established model allergen for airway hyperresponsiveness). The BioLegend LEGEND MAX™ Mouse OVA specific IgE ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with OVA. This kit is specifically designed for the accurate quantitation of mouse OVA specific IgE from cell culture supernatant, serum, plasma, and other biological fluids. It is analytically validated with ready-to-use reagents.
5precoatedplates
Verified Reactivity: Mouse
Application: ELISA
Application References(PubMed link indicates BioLegend citation): Miller M, et al. 2014. J. Immunol. 192:3475. PubMed Nau F, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 308:191. PubMed
Product Citations: Lee H, et al. 2017. Exp Lung Res. 10.1080/01902148.2017.1339141. PubMed Nguyen TV, et al. 2022. Int J Mol Sci. 23: . PubMed Zhou J, et al. 2021. Environ Toxicol. 36:177. PubMed Cho S, et al. 2016. J Exp Med. 213: 235 - 249. PubMed Seo YS, et al. 2019. Sci Rep. 9:2312. PubMed Woo YD, et al. 2020. J Allergy Clin Immunol. . PubMed Le H, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.06.038. PubMed Nakajima T, et al. 2021. J Exp Med. 218:. PubMed Fakih D, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L1333 - L1343. PubMed Miller M, et al. 2014. J Immunol. 192:3475. PubMed Xu H, et al. 2019. Exp Ther Med. 1.278472222. PubMed Li C, et al. 2016. PLoS One. 11: 0161164. PubMed Nau F, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 308:191. PubMed Ren J, et al. 2015. PLoS One. 10: e0140018. PubMed Ding FX, et al. 2018. Pediatr Res. 84:125. PubMed Li X, et al. 2020. Open Med (Wars). 15:483. PubMed
Sensitivity: 20.7 pg/mL
Standard Range: 0.156-10 ng/mL
Gene ID: NA
UniProt: View information about OVA-IgE on UniProt.org
Regulatory Status: RUO
Other Names: OVA-IgE Precoated ELISA Kit
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.