Biolegend, 440008, LEGEND MAX™ Human Myeloperoxidase ELISA Kit, 5precoatedplates

Myeloperoxidase (MPO) is a peroxidase enzyme which is expressed in neutrophils and macrophages. MPO is a 150 kD dimeric protein which consists of two 15 kD light chains and two variable weight glycosylated heavy chains bound to a prosthetic heme group. Three isoforms have been identified, differing in the size of the heavy chain. Mature human MPO shares 88% amino acid sequence identity with dog, mouse, and rat. Human MPO also shares 71%, 56%, and 47% amino acid sequence identity with comparable regions of human eosinophil peroxidase, lactoperoxidase, and thyroid peroxidase, respectively. MPO is released extracellularly, and it has been detected in a wide range of acute and chronic inflammatory conditions. Serum levels of myeloperoxidase (MPO) are associated with coronary artery disease and indicate a risk factor for pancreatic cancer. The LEGEND MAX™ Human Myeloperoxidase ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) is a 96-well strip plate, pre-coated with a monoclonal capture antibody. This kit is specifically designed for the accurate quantitation of human MPO from cell culture supernatant, serum, plasma, saliva, urine and other biological fluids. This kit is analytically validated with ready-to-use reagents.
5precoatedplates
Verified Reactivity: Human
Application: ELISA
Product Citations: Krinsky N, et al. 2023. J Thromb Haemost. . PubMed Li X, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:1077. PubMed Hirao H, et al. 2023. J Clin Invest. 133: . PubMed Bonneau S, et al. 2022. Cells. 11:. PubMed Hertwig L, et al. 2016. Mult Scler. 22: 160 - 173. PubMed LaSalle TJ, et al. 2022. Cell Rep Med. 3:100779. PubMed Chen Z, et al. 2021. Front Med (Lausanne). 8:693605. PubMed
Sensitivity: 28 ± 8 pg/mL
Standard Range: 0.16-10 ng/mL
Biology Area: Cancer Biomarkers, Cell Biology
Molecular Family: Enzymes and Regulators
Gene ID: 4353
UniProt: View information about MPO on UniProt.org
Regulatory Status: RUO
Other Names: MPO, Myeloperoxidase
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.