Biolegend, 445804, ELISA MAX™ Deluxe Set Human IL-1α, 5plates

Please contact our sales representative for bulk pricing quotations. IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1 binds to the cell surface type I and II IL-1 receptors (IL-1RI and IL-1RII). IL-1α, IL-1β, and IL-1RA can compete for binding to these receptors. IL-1 is a pyrogen, and it is an activating factor for lymphocytes. It also damages joints and influences liver proteins. The BioLegend ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). A human IL-1α specific mouse monoclonal antibody is first coated on a 96-well plate. Standards and samples are added to the wells, and IL-1α binds to the immobilized capture antibody. Next, a biotinylated goat polyclonal anti-human IL-1α detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of IL-1α present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.
5plates
Verified Reactivity: Human
Application: ELISA
Product Citations: Rademacher F, et al. 2018. J Innate Immun. 11:125. PubMed Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed Tang Y, et al. 2021. Immunity. 54(3):454-467.e6. PubMed Westcott JM, et al. 2020. Cancer Res. 3933:80. PubMed Wang X, et al. 2021. Front Pharmacol. 12:673818. PubMed
Sensitivity: 0.6 pg/mL
Standard Range: 1.3-80 pg/mL
Cell Sources: Macrophage
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Gene ID: 3552
UniProt: View information about IL-1alpha on UniProt.org
Regulatory Status: RUO
Other Names: Interleukin-1α, LAF, EP, LEM, MCF/MNCF, IL1α
Q: Do the ELISA MAX™ Deluxe Sets come with plates?
A: No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.