Biolegend, 448507, LEGEND MAX™ Mouse M-CSF ELISA Kit, 1precoatedplate

As a potent hematopoietic cytokine, M-CSF is involved in diverse processes, such as the regulation of atherosclerosis or brain, bone resorption, placental development, and inflammatory responses. M-CSF (macrophage colonystimulating factor) is a potent lineage restricted hematopoietic growth factor and member of the CSF family of hormone-like glycoproteins. It plays a key role in the regulation of cellular undifferentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells. BioLegend’s LEGEND MAX™ Mouse M-CSF ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate. The plate is pre-coated with a monoclonal capture antibody. The detection antibody is a biotinylated monoclonal antibody. This kit is specifically designed for the accurate quantitation of mouse M-CSF in cell culture supernatant, serum, and plasma. It is analytically validated with ready-to-use reagents.
1precoatedplate
Verified Reactivity: Mouse
Application: ELISA
Sensitivity: 6.1 ± 1.7 pg/mL
Standard Range: 31.25 – 2000 pg/mL
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area: Cell Biology, Cell Proliferation and Viability, Immunology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Gene ID: 12977
UniProt: View information about M-CSF on UniProt.org
Regulatory Status: RUO
Other Names: colony stimulating factor 1 (macrophage), CSF1, CSF-1, Lanimostim, MCSF, M-CSF
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.