Biolegend, 500104, Purified anti-human IL-1α Antibody, 500μg

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. The 364-3B3-14 antibody reacts with human interleukin-1α (IL-1α). The 364-3B3-14 antibody is a non-neutralizing antibody.
500μg
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human IL-1α
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1α recombinant protein ranging from 200 to 1.56 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA or ELISPOT Capture1: The purified 364-3B3-14 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5313 antibody (Cat. No. 531304) as the detecting antibody and recombinant human IL-1a (Cat. No. 570009) as the standard. The Ultra-LEAF™ purified antibody (Cat. No. 500108) is suggested for ELISPOT capture. Flow Cytometry4: The fluorochrome-labeled 364-3B3-14 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1a -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Additional reported applications (for the relevant formats) include: immunohistochemistry3. The 364-3B3-14 is a non-neutralizing antibody.
Application References(PubMed link indicates BioLegend citation): Thorpe R, et al. 1988. Lymphokine Res. 2:119. Prussin C, et al. 1995. J. Immunol. Meth. 188:117. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. Enriquez, J, et al. 2002. Adv. Perit Dial. 18:177. Lu Y, et al. 2015. PNAS. 112:607. PubMed
Product Citations: Lu Y, et al. 2015. Proc Natl Acad Sci U S A. 112:607. PubMed Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
RRID: AB_315083 (BioLegend Cat. No. 500104)
Structure: Cytokine; 17.5 kD (Mammalian)
Bioactivity: Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia
Cell Sources: Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
Cell Targets: B cells, T cells, monocytes, dendritic cells
Receptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Bomford, R., et al. Eds. 1989. Interleukin-1, inflammation and disease. Elsevier, New York. 3. Brazel, D., et al. 1991. Biotechnol. Ther. 2:241. 4. Dinarello, C. 1996. Blood 87:2095.
Regulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID: 3552
UniProt: View information about IL-1alpha on UniProt.org
Clone: 364-3B3-14
Regulatory Status: RUO
Other Names: Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF/MNCF)
Isotype: Mouse IgG1, κ