Biolegend, 500605, PE anti-human IL-3 Antibody, 25tests

IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The BVD3-1F9 antibody can neutralize the bioactivity of natural or recombinant IL-3.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Yeast-expressed, recombinant human IL-3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: ELISA1-3,6 or ELISPOT4 Detection: The biotinylated BVD3-1F9 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified BVD8-3G11 (Cat. No. 500502) antibody as the capture antibody. Flow Cytometry: The fluorochrome-labelled BVD3-1F9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-3 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Additional reported applications (for the relevant formats) include: immunoprecipitation6, Western blotting6, neutralization1,6, immunohistochemical staining5,7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Application References(PubMed link indicates BioLegend citation): Abrams J, et al. 1992. Immunological Reviews 127:5. Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133. Abrams J. 1995. Curr. Prot. Immunol.. 6.20. Mahanty S, et al. 1992. J. Immunol. 148:3567. Andersson U, et al. 1993. Detection and quantification of gene expression. New York: Springer-Verlag. Kaushansky K. 1992. J. Clin. Invest. 90:1879. Andersson J, et al. 1994. Immunology 83:16.
RRID: AB_2123712 (BioLegend Cat. No. 500606)
Structure: Cytokine; 14-30 kD (Mammalian)
Bioactivity: Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase
Cell Sources: Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells
Cell Targets: Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
Receptors: IL-3R, α subunit (CD123), common β subunit (CDw131)
Biology Area: Cell Biology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Frendl G. 1992. Int. J. Immunopharmacol. 14:421. 3. Ihle J. 1992. Chem. Immunology 51:65.
Regulation: Upregulated by T cell stimulation; downregulated by glucocorticoids
Gene ID: 3562
UniProt: View information about IL-3 on UniProt.org
Clone: BVD3-1F9
Regulatory Status: RUO
Other Names: Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.