Biolegend, 500701, Purified anti-human IL-4 Antibody, 50μg

IL-4 is a pleiotropic cytokine that is produced by activated T cells mast cells and basophils. IL-4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4 + T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10 and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells. The 8D4-8 antibody can neutralize the bioactivity of natural or recombinant IL-4.
50μg
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Yeast-expressed, recombinant human IL-4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested ELISPOT Capture, Neut, ICFC, IHC, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The purified 8D4-8 has been tested by blocking fluorochrome conjugated 8D4-8 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody (≤ 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: ELISPOT Capture, neutralization, intracellular staining for flow cytometry, immunohistochemical staining, and immunoprecipitation.
Application References(PubMed link indicates BioLegend citation): Bird, C., et al. 1991. Cytokine. 3:562. Prussin, C., et al. 1995. J. Immunol. Meth. 188:117. Hickie, I., et al. 2001. Twin Res. 4:94. Heeger, P., et al. 1999. J. Immunol. 163:2267. Jason, J., et al. 1999. Clin. Diagn. Lab Immunol. 6:73. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. Gong, G, et al. 2009. Blood 113:837. PubMed Yoshino, N., et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations: Meckiff BJ, et al. 2019. J Immunol. 203:1276. PubMed Alroqi F, et al. 2017. J Clin Immunol. . 10.1007/s10875-017-0451-1. PubMed Harshe RP, et al. 2020. Nat Commun. 11:5894. PubMed Kameda M, et al. 2020. PLoS One. 15:e0241719. PubMed Yan J, et al. 2020. Cell Rep. 107820:31. PubMed Kurtaj A, et al. 2017. J Allergy Clin Immunol. 140:1746. PubMed
RRID: AB_315113 (BioLegend Cat. No. 500701) AB_315114 (BioLegend Cat. No. 500702)
Structure: Cytokine; 15-19 kD (Mammalian)
Bioactivity: Differentiation of naïve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources: Mast cells, T cells, bone marrow stromal cells
Cell Targets: B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors: Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Boulay, J., et al. 1992. Curr. Opin. Immunol. 4:294. 3. Dullens, H., et al. 1991. In vivo 5:567. 4. Paul, W. 1991. Blood. 77:1859.
Regulation: Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID: 3565
UniProt: View information about IL-4 on UniProt.org
Clone: 8D4-8
Regulatory Status: RUO
Other Names: Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
Isotype: Mouse IgG1, κ