Biolegend, 502001, Biotin anti-human IL-13 Antibody, 50μg

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 lymphocytes. IL-13 shares 30% amino acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include: suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion. The poly5020 polyclonal antibody reacts with human interleukin-13 (IL-13).
50μg
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Recombinant human IL-13
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.2% BSA and 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection, ELISPOT Detection
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. As a detection antibody in ELISA assays, dilute 60 µl of this biotinylated antibody into 12 ml of detection buffer for one 96 well microtiter plate assay. To obtain a linear standard curve, serial dilutions of human IL-13 recombinant protein ranging from 2000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Detection or ELISPOT Detection: The biotinylated Poly5020 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES10-5A2 (Cat. No. 501902/501910) antibody as the capture antibody. This product may contain other non-IgG subtypes.
Additional Product Notes: This antibody can be used for 4 plates.
Application References(PubMed link indicates BioLegend citation): Bailer, R.T., et al.., 1999. J. Immunol.. 162 :7534. Arva, E., et al.., 1999. Scand. J. Immunol.. 49:411.
Product Citations: Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
RRID: AB_2124285 (BioLegend Cat. No. 502001)
Structure: Cytokine; 12.5 kD
Function: Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
Bioactivity: Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
Cell Sources: Activated T helper cells, CD8+ T cells, mast cells, NK cells
Cell Targets: B cells, monocytes, macrophages, basophils
Receptors: Moderate affinity IL-13Rα1; high affinity heterodimer IL-13Rα1/IL-4Rα also binds IL-4; both receptor complexes have γ-subunit in common with IL-2R, IL-4R, IL-7R, IL-15R ; high affinity IL-13Rα2 incapable of signaling
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Hilton, D., et al. 1996. P. Natl. Acad. Sci. USA 93:497. 3. Obiri, N., et al. 1995. J. Biol. Chem. 270:8797. 4. Ochensberger, B., et al. 1996. Blood 88:3028.
Regulation: IL-13Rα2 possible antagonist
Gene ID: 3596
UniProt: View information about IL-13 on UniProt.org
Clone: Poly5020
Regulatory Status: RUO
Other Names: Interleukin-13, NC30
Isotype: Rabbit Polyclonal IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.