Biolegend, 502101, Purified anti-human G-CSF Antibody, 50μg

Granulocyte - colony stimulating factor (G-CSF) is a potent stimulator of bone marrow cells especially those of neutrophilic granulocyte lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-alpha or IFN-gamma.
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli-expressed, recombinant human G-CSF
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality testedIHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA or ELISPOT Capture1,2: The purified BVD13-3A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD11-37G10 antibody (Cat. No. 506702) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Application References(PubMed link indicates BioLegend citation): Abrams J. 1995. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.20. Abrams J, et al. 1992. Immunol. Rev. 127:5. Andersson J, et al. 1994. Immunology 83:16. Sander B, et al. 1991. Immunol. Rev. 119:65. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
Product Citations: Hollmén M, et al. 2016. Oncoimmunology. e1115177:5. PubMed
RRID: AB_315207 (BioLegend Cat. No. 502101) AB_2083733 (BioLegend Cat. No. 502102)
Structure: Cytokine; 21 kD (Mammalian)
Bioactivity: Growth/differentiation/activating factor for neutrophils; proliferation/migration endothelial cells cells; synergistic with IL-3 growth of hematopoietic progenitors
Cell Sources: Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, bone marrow stromal cells
Cell Targets: Hematopoietic progenitors of neutrophil/granulocyte lineage, endothelial cells
Receptors: CD114 (G-CSFR)
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Demetri G, et al. 1991. Blood 78:2791. 3. Moore M. 1991. Annu. Rev. Immunol. 9:159. 4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.
Regulation: Upregulated by IL-1, IL-17, TNF-α, IFN-γ, GM-CSF; downregulated by prostaglandin E2
Gene ID: 1441
UniProt: View information about G-CSF on UniProt.org
Clone: BVD13-3A5
Regulatory Status: RUO
Other Names: Granulocyte-Colony Stimulating Factor, CSF-β, Pluripoietin, Granulocytic neutrophil colony stimulating activity (G-CSA), Macrophage-granulocyte inducer-2 (MGI-2)
Isotype: Rat IgG1, κ