Biolegend, 502302, Purified anti-human GM-CSF Antibody, 500μg

Granulocyte/macrophage - colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.
500μg
Verified Reactivity: Human
Reported Reactivity: Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli-expressed, recombinant human GM-CSF.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested ICFC, IP, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. The purified BVD2-21C11 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated BVD2-23B6 antibody as the detecting antibody, a concentration range of 1-4 µg/ml (BVD2-21C11) is recommended. For flow cytometric application, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. The purified BVD2-21C11 has been tested by blocking fluorochrome conjugated BVD2-21C11 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody (≤ 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: ELISA1-4 or ELISPOT3,4 Detection, Neutralization1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated5,6 and acetone-fixed7 frozen tissue sections, and immunocytochemistry. Note: For testing human GM-CSF in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 432001 to 432006) are specially developed and recommended.
Application References(PubMed link indicates BioLegend citation): Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut, IP) Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133. (ELISA) Bacchetta R, et al. 1990. J. Immunol. 144:902. (ELISA) Kita H, et al. 1991. J. Exp. Med. 174:745. (ELISA) Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC) Andersson J, et al. 1994. Immunology 83:16. (IHC) Rasouli J, et al. 2015. J. Immunol. 11:5085-93. (IHC)
Product Citations: Meckiff BJ, et al. 2019. J Immunol. 203:1276. PubMed Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed Younis R, et al. 2016. J Immunol. 196: 1419 - 1429. PubMed Kim ML, et al. 2018. Circulation. 138:2648. PubMed
RRID: AB_315215 (BioLegend Cat. No. 502301) AB_315216 (BioLegend Cat. No. 502302)
Structure: Cytokine; 22 kD (Mammalian)
Bioactivity: Growth/development factor for granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Cell Sources: T cells, macrophages, fibroblasts, endothelial cells, mast cells
Cell Targets: Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors: Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131), which is also shared with the IL-3 and IL-5 receptor α chains
Biology Area: Cell Biology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Demetri G, et al. 1991. Blood 78:2791. 3. Fan D, et al. 1991. In vivo 5:571. 4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.
Regulation: Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Gene ID: 1437
UniProt: View information about GM-CSF on UniProt.org
Clone: BVD2-21C11
Regulatory Status: RUO
Other Names: Granulocyte/macrophage colony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
Isotype: Rat IgG2a, κ