Biolegend, 503212, Ultra-LEAF™ Purified anti-mouse IL-1α Antibody, 100mg

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. The ALF-161 antibody reacts with precursor, secreted and membrane-associated forms of mouse IL-1α. The ALF-161 antibody can neutralize the bioactivity of natural or recombinant IL-1α.
100mg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: E. coli - expressed, recombinant mouse IL-1
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: ELISA Capture - Quality testedELISPOT Capture, Neut, ICFC, WB, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1.0 - 4.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-1α recombinant protein ranging from 250 to 2 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA or ELISPOT Capture: The purified ALF-161 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5034 antibody (Cat. No. 503401) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. ELISA Capture: The purified ALF-161 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated BL1a-89 antibody (Cat. Nos. 512503 & 512504) as the detection antibody and recombinant mouse IL-1a (Cat. No. 580309) as the standard. Neutralization: Clone ALF-161 has been described to have IL-1a neutralizing properties based on the in vivo mice model3. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended (Cat. No. 503207-503212).
Application References(PubMed link indicates BioLegend citation): Fuhlbrigge R, et al. 1988. J. Immunol. 141:2643. Rogers HW, et al. 1992. P. Natl. Acad.Sci. USA 89:1011. Yazdi AS, et al. 2010. P. Natl. Acad. Sci. USA 107:19449. (Neut)
Product Citations: Malireddi RKS, et al. 2022. Front Immunol. 13:1068230. PubMed
RRID: AB_2861033 (BioLegend Cat. No. 503207) AB_2861034 (BioLegend Cat. No. 503208) AB_2861035 (BioLegend Cat. No. 503209) AB_2861036 (BioLegend Cat. No. 503210) AB_2861037 (BioLegend Cat. No. 503211) AB_2876737 (BioLegend Cat. No. 503212)
Structure: Cytokine; 17.4 kD (Mammalian)
Bioactivity: Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
Cell Sources: Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,
Cell Targets: B cells, T cells, monocytes, fibroblasts
Receptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York. 3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241. 4. Dinarello C. 1996. Blood 87:2095.
Regulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID: 16175
UniProt: View information about IL-1alpha on UniProt.org
Clone: ALF-161
Regulatory Status: RUO
Other Names: Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF/MNCF)
Isotype: Armenian Hamster IgG
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.