Biolegend, 504701, Purified anti-mouse IL-9 Antibody, 50μg

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones. The D8402E8 antibody reacts with mouse IL-9.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Baculovirus-expressed, recombinant mouse IL-9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-9 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Capture1,2: The purified D8402E8 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated D9302C12 antibody (Cat. No. 504804) as the detecting antibody.
Application References(PubMed link indicates BioLegend citation): Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. Renauld, J., et al. 1995. J. Leuk. Biol. 57:353. Gounni, A.S.,et al.2004. J. Immunol.173:2771.J.Immunol
Product Citations: Gounni A, et al. 2004. J Immunol. 173:2771. PubMed
RRID: AB_315344 (BioLegend Cat. No. 504702)
Structure: Cytokine; 30-40 kD (Mammalian)
Bioactivity: Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Cell Sources: IL-2 activated TH2 lymphocytes
Cell Targets: T lymphocytes, erythroid precursors
Receptors: IL-9R
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Quesniaux, V. 1992. Research Immunology 143:385. 3. Renauld, J., et al. 1993. Adv. Immunol. 54:79. 4. Yang, Y. 1992. Leuk. Lymphoma 8:441.
Regulation: Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Gene ID: 16198
UniProt: View information about IL-9 on UniProt.org
Clone: D8402E8
Regulatory Status: RUO
Other Names: Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Isotype: Rat IgG2a, κ