Biolegend, 505211, PerCP/Cyanine5.5 anti-mouse IL-12/IL-23 p40 (monomer, dimer, heterodimer) Antibody, 25μg

The C15.6 antibody reacts with mouse IL-12 p40 subunit of the IL-12 p70 and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer, or heterodimer. The C15.6 antibody can not neutralize the bioactivity of natural or recombinant IL-12.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CHO-expressed, recombinant mouse IL-12 p70
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: ELISA or ELISPOT Capture1,2,4,6,8,10: The purified C15.6 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated C17.8 (Cat. No. 505302) antibody as the detecting antibody. This assay detects p40 as monomer, homodimer, or heterodimer complexed with p35. The LEAF™ purified antibody is suggested for ELISPOT capture.Additional reported applications (for the relevant formats) include: immunohistochemical staining9 of paraformaldehyde-fixed saponin-treated frozen tissue sections, immunoprecipitation3, and Western blotting3.Note: For testing mouse IL-12 p40 (monomer, dimer, heteromer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431601 to 431606) are specially developed and recommended.
Application References(PubMed link indicates BioLegend citation): Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260. Reichmann G, et al. 1999. J. Immunol. 163:3354. Wysocka M, et al. 1995. Eur. J. Immunol. 25:672. Gately M. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.16. Macatonia S, et al. 1995. J. Immunol. 154:5071. O'Connell PJ, et al. 2006. Blood 107:1010. Akilov OE, et al. 2007. J. Leukocyte Biol. 2007;10.1189/jlb.0706439. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. Huang LY, et al. 2001. J. Immunol. 167:1423. Xu G, et al. 2007. J. Immunol. 179:5358. PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Malu DT, et al. 2011. J. Immunol. 186:6271. PubMed
Product Citations: Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed Weinstock NI, et al. 2020. Neuron. 107(1):65-81. PubMed
RRID: AB_2566224 (BioLegend Cat. No. 505211) AB_2566225 (BioLegend Cat. No. 505212)
Structure: Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity: IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me
Cell Sources: Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets: T cells, NK cells
Receptors: IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Quesniaux V. 1992. Research Immunol. 143:385. 3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355. 4. Trinchieri G, et al. 1993 Immunol. Today. 14:335. 5. Oppmann B, et al. 2000 Immunity. 13:715. 6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910. 7. Parham C, et al. 2002 J. Immunol. 168:5699. 8. Belladonna ML, et al. 2002 J. Immunol. 168:5448. 9. Lankford, CS, et al. 2003 J. Leukocyte Biol. 73:49.
Regulation: Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Gene ID: 16160
UniProt: View information about IL-12/IL-23 p40 on UniProt.org
Clone: C15.6
Regulatory Status: RUO
Other Names: Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
Isotype: Rat IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.