Biolegend, 505910, APC anti-mouse/rat/human MCP-1 Antibody, 100μg

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reacts with mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of natural or recombinant MCP-1.
100μg
Verified Reactivity: Mouse, Rat, Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Hamster
Immunogen: CHO-expressed, recombinant mouse MCP-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: ELISA or ELISPOT Capture1: The Ultra-LEAF™ 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1. Flow Cytometry2: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization1,4,6: The Ultra-LEAF™ purified antibody (Cat. Nos. 505911 & 505912) is recommended for neutralization. Additional reported applications (for the relevant formats) include: Western blotting3, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections5, and acetone-fixed frozen tissue sections7.
Application References(PubMed link indicates BioLegend citation): Luo, Y., et al. 1994. J. Immunol. 153:3708. Zhang, Y., et al. 2002. J. Immunol. 168:3088. Luo, Y., et al. 1999 J. Immunol. 163:3985. Morrison, B. E., et al. 2003 J. Clin. Invest. 112:1862. Hancock, W. W., et al. 1997 Transplantation 64:696. Yu, R., et al. 2006 Obesity 14:1353. Taal MW, et al. 2001. Am. J. Physiol. Renal. Physiol. 280:F343. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed
Product Citations: Campisi L, et al. 2022. Nature. 606:945. PubMed Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed Pascual–García M, et al. 2019. Nat Commun. 10:2416. PubMed
RRID: AB_2566053 (BioLegend Cat. No. 505909) AB_2566054 (BioLegend Cat. No. 505910)
Structure: CC chemokine; dimer; 13.8 kD (Mammalian)
Bioactivity: Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Cell Sources: Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Cell Targets: Monocytes, basophils, T cells, NK cells, immature dendritic cells
Receptors: CCR2; CCR5; CCR10; D6; possibly L-CCR
Biology Area: Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Bischoff, S., et al. 1992. J. Exp. Med. 175:1271. 3. Charo, I., et al. 1994. P. Natl. Acad. Sci. USA 91:2752. 4. Jiang, Y., et al. 1992. J. Immunol. 148:2423.
Regulation: Upregulated by IL-1, TNF-α, PDGF, TGF-β
Gene ID: 20296247706347
UniProt: View information about CCL2 on UniProt.org
Clone: 2H5
Regulatory Status: RUO
Other Names: Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2
Isotype: Hamster IgG