Biolegend, 506601, Biotin anti-human IL-7 Antibody, 50μg

IL-7 is a potent lymphoid cell growth factor which promotes the proliferation of pre-B cells, thymocytes, T cell progenitors, and mature T cells. IL-7 induces formation of LAK cells and CTLs, and can stimulate tumoricidal activity of monocytes and macrophages. IL-7 can induce upregulation of proinflammatory cytokines in myeloid cells. IL-7 is expressed by stromal cells. The BVD10-11C10 monoclonal antibody is specific for human interleukin-7 (IL-7).
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli - expressed, recombinant human IL-7
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection, ELISPOT Detection
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-7 recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 2-8 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA or ELISPOT Detection: The biotinylated BVD10-11C10 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified BVD10-40F6 antibody (Cat. No. 501302/501304) as the capture antibody.Additional reported applications (for the relevant formats) include: The BVD10-11C10 is a non-neutralizing antibody.
Application References(PubMed link indicates BioLegend citation): Abrams J, et al. 1992. Immunol. Rev. 127:5. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons New York.
Product Citations: Goyal G, et al. 2022. Adv Sci (Weinh). 9:e2103241. PubMed Haug V, et al. 2020. Transplantation. e260:104. PubMed Gilboa T, et al. 2020. Adv Healthc Mater. e2001111:. PubMed
RRID: AB_2296031 (BioLegend Cat. No. 506601) AB_2280326 (BioLegend Cat. No. 506602)
Structure: Cytokine; 20-28 kD (Mammalian)
Bioactivity: Proliferation of pre-/pro-B cells, thymocytes, T cells; formation of LAK cells, CTLs, stimulation of tumoricidal activity monocytes/macrophages; upregulate proinflammatory cytokines in myeloid cells
Cell Sources: Adherent bone marrow stromal cells, thymic stromal cells, spleen, keratinocytes
Cell Targets: Progenitor B and T cells; CD4-CD8- thymocytes; megakaryocytes
Receptors: Heterodimer IL-7Rα (CD127); γ-subunit (CD132) in common with IL-2R, IL-4R, IL-13R, IL-15R
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Park L, et al. 1992. Adv. Exp. Med. Biol. 323 125. 3. Melchers F, et al. 1992. Adv. Exp. Med. Biol. 323 111.
Regulation: Downregulated by TGF-β
Gene ID: 3574
UniProt: View information about IL-7 on UniProt.org
Clone: BVD10-11C10
Regulatory Status: RUO
Other Names: Interleukin-7, Lymphopoietin-1 (LP-1), Pre-B cell growth factor, Thymocyte growth factor
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.