Biolegend, 507205, PE anti-human Granzyme A Antibody, 25tests

Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human Granzyme A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2.
Application References(PubMed link indicates BioLegend citation): Trimble L, et al. 1998. Blood 91:585. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285. Raqib R, et al. 2002. Infect. Immun. 70:3199. Chen H, et al. 2005. J. Immunol. 175:591.
Product Citations: Shokri MR, et al. 2019. Sci Rep. 9:10007. PubMed Ramaswamy A, et al. 2021. Immunity. 54(5):1083-1095.e7. PubMed Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
RRID: AB_315472 (BioLegend Cat. No. 507206)
Structure: Serine protease; disulfide-linked homodimer; 28 kD (Mammalian)
Bioactivity: Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking
Cell Sources: Cytotoxic T cells, NK cells
Cell Targets: Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1
Receptors: Assisted by perforin
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Enzymes and Regulators, Proteases
Antigen References: 1. Brune J, et al. 1986. Nature 322:268. 2. Fan Z, et al. 2003. Nature Immunol. 4:145. 3. Fan Z, et al. 2003. Cell 112:659. 4. Masson D, et al. 1987. Cell 49:679.
Gene ID: 3001
UniProt: View information about Granzyme A on UniProt.org
Clone: CB9
Regulatory Status: RUO
Other Names: Granzyme 1, Hanukah factor serine protease (HFSP), Cytotoxic T-lymphocyte-associated serine esterase 3 (CTLA-3)
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.