Biolegend, 507218, Alexa Fluor® 594 anti-human Granzyme A Antibody, 100μg

Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human Granzyme A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2.
Application References(PubMed link indicates BioLegend citation): Trimble L, et al. 1998. Blood 91:585. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285. Raqib R, et al. 2002. Infect. Immun. 70:3199. Chen H, et al. 2005. J. Immunol. 175:591.
RRID: AB_2563964 (BioLegend Cat. No. 507218)
Structure: Serine protease; disulfide-linked homodimer; 28 kD (Mammalian)
Bioactivity: Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking
Cell Sources: Cytotoxic T cells, NK cells
Cell Targets: Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1
Receptors: Assisted by perforin
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Enzymes and Regulators, Proteases
Antigen References: 1. Brune J, et al. 1986. Nature 322:268. 2. Fan Z, et al. 2003. Nature Immunol. 4:145. 3. Fan Z, et al. 2003. Cell 112:659. 4. Masson D, et al. 1987. Cell 49:679.
Gene ID: 3001
UniProt: View information about Granzyme A on UniProt.org
Clone: CB9
Regulatory Status: RUO
Other Names: Granzyme 1, Hanukah factor serine protease (HFSP), Cytotoxic T-lymphocyte-associated serine esterase 3 (CTLA-3)
Isotype: Mouse IgG1, κ