Biolegend, 507803, FITC anti-rat IFN-γ Antibody, 25tests

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The DB-1 antibody reacts with rat and mouse interferon-gamma (IFN-γ). The DB-1 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The DB-1 antibody has been well characterized for ELISPOT, ELISA, intracellular staining, Western blotting, IHC, and neutralization ( in vitro and in vivo ).
25tests
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant rat IFN-γ
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: ELISA Capture1 or ELISPOT Capture2: The purified DB-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated poly5109 antibody (Cat. No. 510901) as the detecting antibody and recombinant IFN-γ (Cat. No. 565701) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture. Flow Cytometry5: The fluorochrome-labeled DB-1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization3,4: The LEAF™ purified antibody (Endotoxin in vivo and in vitro (Cat. No. 507808).Additional reported applications (for the relevant formats) include: Western blotting1, and immunohistochemistry2 of paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Application References(PubMed link indicates BioLegend citation): Van der Meide P, et al. 1989. Lymphokine Res. 8:439. Nennesmo I, et al. 1989. Brain Res. 504:306. Rayner D, et al. 1987. Scand. J. Immunol. 25:621. Hartung H, et al. 1990. Ann Neurol. 27:247. Bernard I, et al. 1998. Eur. Cytokine Net. 9:613.Nakagawa P, et al. 2012. Am J Physiol Heart Circ Physiol. 303:1114. PubMedChao GY, et al. 2014. J. Immunol. 192:3645. PubMed
Product Citations: Cho KH, et al. 2022. Front Immunol. 13:930511. PubMed Amarnath S, et al. 2011. PLoS One. 6:e18885. PubMed Ding Y, et al. 2016. J Immunol. 197: 3831 - 3840. PubMed Chao G, et al. 2014. J Immunol. 192:3645. PubMed Nakagawa P, et al. 2012. Am J Physiol Heart Circ Physiol. 303:1114. PubMed
RRID: AB_315495 (BioLegend Cat. No. 507804)
Structure: Cytokine; dimer; 40-80 kD (Mammalian)
Bioactivity: Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources: CD8+ and CD4+ T cells, NK cells
Cell Targets: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type: Tregs
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321. 3. Farrar M, et al. 1993. Annu .Rev. Immunol. 11:571. 4. Gray P, et al. 1987. Lymphokines 13:151.
Regulation: Upregulated by IL-2, bFGF, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Gene ID: 25712
UniProt: View information about IFN-gamma on UniProt.org
Clone: DB-1
Regulatory Status: RUO
Other Names: Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype: Mouse IgG1, κ