Biolegend, 508701, Biotin anti-human FGF-basic Antibody, 50μg

Fibroblast growth factor-basic (FGF-b, FGF-2) is a heparin-binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-basic also exerts a potent angiogenic activity in vivo . FGF-basic has been isolated from neural, pituitary, adrenal cortex, and placental tissues. The JKFb-2 antibody reacts with human fibroblast growth factor - basic (FGF-basic).
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human bFGF
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human FGF-basic protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate.It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Detection: The biotinylated JKFb-2 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified BHFB15 antibody (Cat. No. 514801/514802) as the capture antibody.
RRID: AB_315527 (BioLegend Cat. No. 508702)
Structure: FGF; 17.2 kD (Mammalian)
Bioactivity: Modulator of cell proliferation, motility, differentiation, angiogenesis; affinity for heparin; associated with ECM
Cell Sources: Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartilage, endothelial cells, epithelial cells
Cell Targets: Fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, astrocytes, oligodendrocytes, smooth muscle
Receptors: FGFR-1 (flg); FGFR-2 (bek, K-sam); FGFR-4 (flg-2); low affinity coreceptor heparin sulfate and heparin sulfate proteoglycans required for full activity
Biology Area: Angiogenesis, Cell Biology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, Cytokines/Chemokines, Growth Factors
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Klagsbrun M. 1992. Semin. Cancer Biol. 3:81. 3. Goldfarb M. 1990. Cell Growth Differ. 1:439. 4. Sensenbrenner M. 1993. Prog. Neurobiol. 41:683.
Regulation: Released after tissue injuries and during inflammatory processes
Gene ID: 2247
UniProt: View information about FGF-basic on UniProt.org
Clone: JKFb-2
Regulatory Status: RUO
Other Names: Fibroblast Growth Factor-basic, bFGF, FGF-2, Heparin-binding growth factor
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.